Construction of next generation sequencing (NGS) libraries using competitive strand displacement

A sequencing adapter comprising:

• a first DNA strand and a second DNA strand;
• wherein the first DNA strand has a blocking group at its 3′ end and a 5′ phosphate and comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:
• 19, SEQ ID NO:
• 20, SEQ ID NO:
• 21, SEQ ID NO:
• 22, SEQ ID NO:
• 23, SEQ ID NO:
• 24, SEQ ID NO:
• 25, SEQ ID NO:
• 26, SEQ ID NO:
• 27, SEQ ID NO:
• 28, SEQ ID NO:
• 29, SEQ ID NO:
• 30, SEQ ID NO:
• 31, SEQ ID NO:
• 32, and SEQ ID NO:
• 33;
• wherein the second DNA strand has a dideoxy nucleotide base (ddN) at its 3′ end and comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:
• 34, SEQ ID NO:
• 35, SEQ ID NO:
• 36, SEQ ID NO:
• 37, SEQ ID NO:
• 38, SEQ ID NO:
• 39, SEQ ID NO:
• 40, SEQ ID NO:
• 41, SEQ ID NO:42, SEQ ID NO:
• 43, SEQ ID NO:
• 44, SEQ ID NO:
• 45, SEQ ID NO:
• 46, SEQ ID NO:
• 47, SEQ ID NO:
• 48, and SEQ ID NO:
• 49;
• and wherein the second DNA strand is partially complementary to the first DNA strand.