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US 10,894,966 B2Granted
Virus-based expression vectors and uses thereof
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsBernard Moss, Linda S. Wyatt
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Abstract
Expression vectors ideal for use in vaccinating individuals against disease based on vaccinia virus and other chordopoxviruses having high expression of recombinant genes and low expression of vector genes in target animals, and low expression of recombinant genes and high expression of vector genes in cells used for propagation.
Claims33
A recombinant chordopoxvirus comprising:
- a) a first nucleic acid sequence encoding a heterologous DNA-dependent RNA polymerase, wherein the first nucleic acid sequence is functionally linked to a chordopoxvirus pre-replicative promoter;
- b) a second nucleic acid sequence encoding a heterologous repressor protein, wherein the second nucleic acid sequence is functionally linked to a chordopoxvirus post-replicative promoter;
- and,
- c) at least one inactivating mutation in an open reading frame (ORF) required for the expression of post-replicative genes;
- wherein the recombinant chordopoxvirus is capable of replicating its genome.
2 more elements
Dependencies are inferred from the claim text.