US 10,894,964 B2 · Use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants

Abstract

The present invention refers to the use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants. The expression levels of the heat shock protein (Gmhsp17.6-L), when compared in resistant and susceptible individuals in the population, demonstrated that the largest expression levels per quantitative PCR were present in the individuals that contained the largest AT insertions in the promoter region. The invention also refers to gene expression cassettes containing promoter regions of the gene with different numbers of AT insertions fused to the GUS protein, for transforming soybean embryos.

Claims13

Dependent claims

A method for regulating expression levels of coding sequences in plants comprising:

(i) stably transforming a plant cell with an expression cassette comprising a modified promoter element operably linked to a coding sequence of interest,
wherein said modified promoter element is a variant of an unmodified promoter element, said unmodified promoter element having the nucleotide sequence of SEQ ID NO:
21, and wherein said modified promoter element contains between 9 and 33 AT repetitions between the nucleotide positions that correspond to nucleotide positions 114 to 132 of SEQ ID NO:
21;
(ii) culturing the stably transformed plant cell under plant cell growing conditions;
and
(iii) regenerating a transgenic plant having stably incorporated into its genome the cassette of (i).

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/132,006Filed 28 Nov 2008

Published

US 2014/0075594 A113 Mar 2014

Granted

19 Jan 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 15/8285
For biotic stress resistance, pathogen resistance, disease resistance: for nematode resistance
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 15/8237
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: externally regulated expression systems

Use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants

Life of this patent

Use of AT(n) insertions in promoter elements for controlling the expression levels of coding sequences in plants

Life of this patent

Abstract

Claims13