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US 10,894,958 B1Granted
Automated cell processing methods, modules, instruments, and systems
AssigneeInscripta, Inc.
InventorsKevin Ness, Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill
Life of this patent
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Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
Claims20
An automated stand-alone multi-module cell editing instrument for performing nuclease-directed editing in mammalian cells comprising:
- a housing configured to house all or some of the modules;
- a receptacle configured to receive the mammalian cells;
- one or more receptacles configured to receive expression vectors, wherein the expression vectors comprise a viral vector backbone and an editing cassette;
- a growth module for growing cells for transfection;
- a transfection module configured to introduce the expression vectors into the mammalian cells;
- a nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the mammalian cells;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script;
- and
- an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.
6 more elements
An automated stand-alone multi-module cell editing instrument for performing nuclease-directed editing in mammalian cells comprising:
- a housing configured to house all or some of the modules;
- a receptacle configured to receive the mammalian cells;
- one or more receptacles configured to receive expression vectors, wherein the expression vectors comprise a viral vector backbone and an editing cassette;
- a growth module for growing cells for transfection;
- a transfection module configured to introduce the expression vectors into the mammalian cells;
- a nuclease-directed editing module configured to allow the introduced expression vectors to edit nucleic acids in the mammalian cells;
- a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script;
- and
- an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.
6 more elements
Dependencies are inferred from the claim text.