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US 10,894,956 B2Granted
Phospholipid nanogel for exoglycosidase enzyme stabilization
AssigneeWest Virginia University
InventorsLisa Holland, Srikanth Gattu
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Abstract
Described herein are phospholipid nanogels that can contain a low concentration (less than about 250 μUnits/μL) of an exoglycosidase enzyme. Also described herein are systems and devices that can contain a phospholipid nanogel that can contain a low concentration (less than about 250 μUnits/μL) of an exoglycosidase enzyme. Also described herein are methods of using the phospholipid nanogels described herein and devices and systems that can contain a phospholipid nanogel described herein.
Claims5
A phospholipid nanogel comprising:
- a lipid comprising 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) with a ratio of DMPC to DHPC that ranges from 2.0 to 3.0, wherein the lipid is in an amount effective to produce a gel transition temperature of about 23° C., wherein the phospholipid nanogel has a fluid-like viscosity at a temperature range from 4° C. to about 23° C., and wherein the phospholipid nanogel forms a viscous gel from about 23° C. to 60° C.;
- and an amount of an exoglycosidase enzyme that ranges from about 0.001 μUnits/μL of the phospholipid nanogel to about 250 μUnits/μL of the phospholipid nanogel, and
- wherein the pH of the phospholipid nanogel ranges from about 4 to about 8.
Dependencies are inferred from the claim text.