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US 10,894,955 B2Granted
Mutant decarbonylase gene, recombinant microorganism comprising the mutant decarbonylase gene, and method for producing alkane
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Shusei Obata
Life of this patent
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Abstract
The present disclosure is intended to identify a substitution mutation that improves enzyme activity of a decarbonylase. An amino acid corresponding to an amino acid 90, 107, 163, or 171 in SEQ ID NO: 2 is to be substituted with an amino acid exhibiting a higher degree of hydrophobicity.
Claims11
A mutant decarbonylase gene encoding a decarbonylase having at least one substitution mutation in the amino acid sequence as shown in SEQ ID NO:
- 2, wherein the mutation is selected from the group consisting of:
- a substitution mutation of an amino acid corresponding to glycine 90 with an amino acid exhibiting a higher degree of hydrophobicity than that of the amino acid to be substituted;
- a substitution mutation of an amino acid corresponding to serine 163 with an amino acid exhibiting a higher degree of hydrophobicity than that of the amino acid to be substituted;
- and
- a substitution mutation of an amino acid corresponding to asparagine 171 with an amino acid exhibiting a higher degree of hydrophobicity than that of the amino acid to be substituted.
2 more elements
Dependencies are inferred from the claim text.