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US 10,894,954 B2Granted
Subtilisin variants and uses thereof
OwnerFrancis Edward Nanoinventor
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Abstract
The present invention relates to subtilisin variants and uses thereof. More specifically, the present invention relates to variant subtilisin Carlsberg sequences and uses thereof.
Claims20
A variant subtilisin Carlsberg polypeptide, or a processed fragment thereof, the polypeptide comprising a mutation at one or more of amino acid positions K88, D180, N181, N265, L339 or Q379, wherein the amino acid position corresponds to the numbering set forth in SEQ ID NO:2, or combinations thereof, and wherein the polypeptide is heat labile.
A nucleic acid molecule comprising the sequence set forth in any one of SEQ ID NOs:
- 3 to 8 or a fragment thereof, wherein the nucleic acid sequence encodes a polypeptide comprising D180G, L339M, and Q379P substitutions (SEQ ID NO:
- 3 or SEQ ID NO:
- 4), L339M and Q379P substitutions (SEQ ID NO:
- 5 or SEQ ID NO:
- 6), D180A, L339M, and Q379P substitutions (SEQ ID NO:
- 7 or SEQ ID NO:
- 8), wherein the amino acid position number corresponds to the numbering set forth in SEQ ID NO:
- 2.
5 more elements
Dependencies are inferred from the claim text.