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US 10,894,951 B2Granted
Heterologous expression of short-chain monooxygenases in microorganisms
AssigneeIndustrial Microbes, Inc.
InventorsElizabeth Jane Clarke, Baolong Zhu, Derek Lorin Greenfield, Stephanie Rhianon Jones, Noah Charles Helman
Life of this patent
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Abstract
Methods and compositions for the oxidation of short alkanes by engineered microorganisms expressing recombinant enzymes is described, along with methods of use.
Claims12
A synthetic polynucleotide for a soluble diiron monooxygenase enzyme which can be expressed in a microorganism of interest, comprising at least one monooxygenase coding region encoding a diiron monooxygenase enzyme comprising SEQ ID NO:
- 10 and at least one protein folding chaperone coding region encoding at least one protein folding chaperone, the at least one monooxygenase coding region linked to at least one promoter which will function in the microorganism of interest and allow the microorganism of interest to oxidize short chain alkanes and the at least one protein folding chaperone coding region linked to at least one promoter which will function in the microorganism of interest.
Dependencies are inferred from the claim text.