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US 10,894,948 B2Granted
Resetting pluripotent stem cells
AssigneeCambridge Enterprise Limited
InventorsAustin Smith, Ge Guo, Yasuhiro Takashima
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Abstract
The invention provides methods and materials for resetting or sustaining a human stem cell in a “naïve” or “ground” state, based on the use of media including combinations of inhibitors. An example naïve culture medium comprises a PKC inhibitor, a MEK inhibitor. Also provided are methods of obtaining or propagating such cells, cells obtained using these methods, and novel culture media, which can be used in these methods.
Claims18
A method of resetting a human stem cell to a naive state, the method comprising:
- (a) providing a human stem cell to be reset,
- (b) inducing a naive state by
- (i) optionally introducing one or more heterologous reprogramming factors into the cell for expression thereof,
- (ii) culturing the cell in a resetting medium, wherein the resetting medium comprises a MEK inhibitor, a tankyrase inhibitor and a STAT3 activator,
- (c) sustaining the cell in a naive culture medium, wherein the naive culture medium is different from the resetting medium and comprises a MEK inhibitor, a PKC inhibitor, a GSK3 inhibitor, and a STAT3 activator,
- thereby resetting a human stem cell to a naive state.
3 more elements
Dependencies are inferred from the claim text.