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US 10,894,945 B2Granted
Methods for improving proliferation and stemness of limbal stem cells
AssigneeCatholic University Industry Academic Cooperation Foundation
InventorsSo-Hyang Chung, Hyun Jung Lee
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Abstract
Provided are methods for improving the proliferation and stemness of limbal stem cells (LSCs) by adding a Wnt inhibitor to a medium for limbal explant cultures. When human limbal explants are cultured according to the methods, Wnt/β-catenin signaling is inhibited, resulting in improvement of LSC proliferation, and improvement in the stemness of LSCs, and thus the LSCs may be obtained in high yield.
Claims2
A method for improving the proliferation and maintaining the stemness of limbal stem cells, consisting of:
- (a) immersing in a medium a human limbal explant prepared by placing an epithelial part of a limbal tissue face-up on a polyester membrane insert;
- and
- (b) adding a Wnt inhibitor to the medium, thereby improving the proliferation and maintaining the stemness of limbal stem cells,
- wherein the Wnt inhibitor is N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2-d]pyrimidin-2-yl)thio]-acetamide (IWP2) alone.
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Dependencies are inferred from the claim text.