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US 10,894,944 B2Granted
Cell culture media
AssigneeMonash University
InventorsAndrew George Elefanty, Edouard Guy Stanley, Elizabeth Siewsun Ng
Life of this patent
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Abstract
A serum-free media composition for maintenance or directed differentiation of human embryonic stem cells (HESCs) toward particular cell lineages is disclosed. The media comprises one or more cell nutrient media, a recombinant human albumin or equivalent thereof and optionally at least one agent involved in HESC differentiation. The media composition is substantially free of any human or animal derived products.
Claims28
A serum-free cell differentiation media composition, consisting essentially of:
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof;
- and a polymeric alcohol, lactam, an essential fatty acid, a sterol, recombinant human insulin, anti-oxidants, a synthetic form of a naturally occurring amino acid, an antibiotic, transferrin and transferrin substitutes;
- and a trace element selected from the group consisting of Ag+, Al3+, Ba2+, Cd2+, Co2+, Cr3+, Ge4+, Se4+, Br−, I−, Mn2+, F−, Si4+, V5+, Mo6+, Ni2+, Rb+, Sn2+, Zr4+ and combinations thereof, wherein the cell nutrient media is free of human and animal products.
7 more elements
A serum-free cell differentiation media composition, consisting essentially of:
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- a trace element selected from the group consisting of Ag+, Al3+, Ba2+, Cd2+, Co2+, Cr3+, Ge4+, Se4+, Br−, I−, Mn2+, F−, Si4+, V5+, Mo6+, Ni2+, Rb+, Sn2+, Zr4+ and combinations thereof;
- recombinant human insulin, an anti-oxidant;
- a synthetic form of a naturally occurring amino acid, an antibiotic, transferrin or a transferrin substitute, a compound selected from the group consisting of polyvinyl alcohol (PVA), poly ethyleneglycol (PEG) and polyvinylpyrrolidone (PVP);
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof;
- wherein the cell nutrient media is free of human and animal products.
9 more elements
A serum-tree cell differentiation media composition consisting essentially of:
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- a trace element, wherein the trace element is Se4+;
- recombinant human insulin, an anti-oxidant, a synthetic form of a naturally occurring amino acid;
- an antibiotic, transferrin or a transferrin substitute, polyvinyl alcohol (PVA);
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof, wherein the cell nutrient media is free of human and animal products.
8 more elements
A serum-free cell differentiation media composition, consisting of:
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof;
- and a polymeric alcohol, lactam, an essential fatty acid, a sterol, recombinant human insulin, an anti-oxidants, a synthetic form of a naturally occurring amino acid, an antibiotic, transferrin and transferrin substitutes;
- and a trace element selected from the group consisting of Ag+, Al3+, Ba2+, Cd2+, Co2+, Cr3+, Ge4+, Se4+, Br−, I−, Mn2+, F−, Si4+, V5+, Mo6+, Ni2+, Rb+, Sn2+, Zr4+ and combinations thereof, wherein the cell nutrient media is free of human and animal products.
7 more elements
A serum-free cell differentiation media composition, consisting of:
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- a trace element selected from the group consisting of Ag+, Al3+, Ba2+, Cd2+, Co2+, Cr3+, Ge4+, Se4+, Br−, I−, Mn2+, F−, Si4+, V5+, Mo6+, Ni2+, Rb+, Sn2+, Zr4+ and combinations thereof;
- recombinant human insulin, an anti-oxidant, a synthetic form of a naturally occurring amino acid, an antibiotic, transferrin or a transferrin substitute, a compound selected from the group consisting of polyvinyl alcohol (PVA), poly ethyleneglycol (PEG) and polyvinylpyrrolidone (PVP);
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof;
- wherein the cell nutrient media is free of human and animal products.
8 more elements
A serum-free cell differentiation media composition consisting of;
- a cell nutrient media;
- recombinant human albumin;
- sodium;
- octanoate;
- cholesterol;
- linoleic acid;
- linolenic acid;
- a trace element, wherein the trace element is Se4+;
- recombinant human insulin, an anti-oxidant, a synthetic form of a naturally occurring amino acid, an antibiotic, transferrin or a transferrin substitute, polyvinyl alcohol (PVA);
- and a human embryonic stem cell (HESC) differentiation agent which agent directs the differentiation of the HESC towards a cell lineage selected from the group consisting of ectoderm, mesoderm, endoderm and combinations thereof;
- wherein the cell nutrient media is free of human and animal products.
8 more elements
Dependencies are inferred from the claim text.