Two-component bioink, 3D biomaterial comprising the same and method for preparing the same

A two-component bioink for 3D printing, comprising:

• a first solution and a second solution separately,
• wherein the first solution includes a first biopolymer having a first chemical functional group, and the second solution includes a second biopolymer having a second chemical functional group able to chemically bond with the first chemical functional group,
• wherein a combination of the first chemical functional group and the second chemical functional group is tetrazine and cyclooctene, alkyne group and azide group, alkyne group and thiol group, epoxy group and amine group, epoxy group and thiol group, acroyl group and amine group, or acroyl group and thiol group, and
• wherein the first chemical functional group is introduced to the first biopolymer from one or more of the material selected from the group consisting of methyltetrazine-amine, methyltetrazine-PEG4-amine, methyltetrazine-propylamine, tetrazine-PEG5-NHS ester, methyltetrazine-PEG4-NHS ester, methyltetrazine-sulfo-NHS ester, methyltetrazine-PEG4-acid, methyltetrazine-PEG12-NHS ester, methyltetrazine-NHS ester, methyltetrazine-acid, tetrazine-acid, amino-PEG4-alkyne, alkyne-PEG5-acid, alkyne-PEG-amine, oxiranylamine, 2-oxiranyl-ethylamine, acrylamide, acrylic acid and acryloyl chloride, and
• the second chemical functional group is introduced to the second biopolymer from one or more of the material selected from the group consisting of trans-cyclooctene-amine, trans-cyclooctene-NHS ester, trans-cyclooctene-PEG-NHS ester, trans-cyclooctene-PEG4-acid, azide-PEG4-amine, 3-amino-1-propanethiol, 11-mercaptoundecanoic acid, amino-methanethiol, thiol PEG amine, ethylene diamine, PEG diamine, (S)-3-amino-2-(hydroxymethyl)propionic acid and amino-acetic acid.