Selective enrichment of antibodies
AssigneeBoehringer Ingelheim International GmbH
InventorsDorothee Ambrosius, Michael Dieterle, Philine Dobberthien, Maria-Katharina Wiegeshoff
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Abstract
The invention relates to a process for the selective concentration of immunoglobulins or other proteins that contain an Fc domain (target protein), comprising the following steps: a. preparing a solution that contains the target protein; b. incorporating an Fc-binding protein with precisely two binding sites under conditions that allow binding to occur; c. separating the precipitate from the liquid phase; d. undoing the binding of the target protein from the Fc-binding protein.
Claims6
Process for the selective concentration of a target protein which is an immunoglobulin or another protein containing an Fc domain, comprising the following steps:
- a. preparing a solution that contains the target protein;
- b. incorporating an Fc-binding protein with precisely two binding sites under conditions that allow binding to occur;
- c. separating precipitate from liquid phase;
- d. separating the Fc-binding protein from the target protein;
- wherein the Fc-binding protein is a dimer of an Fc-binding domain that comprises one of the sequences represented by SEQ ID NO:1 to SEQ ID NO:11, wherein the Fc-binding protein is not conjugated to a polymer, and wherein the Fc-binding protein is not conjugated to protein A.
Dependencies are inferred from the claim text.