US 10,894,151 B2Granted

Biosensor tattoos and uses therefor for biomarker monitoring

AssigneesCarnegie Mellon University, University of Pittsburgh—Of the Commonwealth System of Higher Education

InventorsO. Burak Ozdoganlar, Marcel P. Bruchez, Phil G. Campbell, Jonathan W. Jarvik, Louis Falo, Geza Erdos

Life of this patent

Estimating the term…

Loading patent…

Abstract

Provided herein are devices and methods used to produce tattoo biosensors that are based on spatially controlled intracutaneous gene delivery of optical reporters driven by specific transcription factor pathways for a given cytokine or other analyte. The biosensors can be specific to a given analyte, or more generically represent the convergence of several cytokines into commonly shared intracellular transcription factor pathways. These biosensors can be delivered as an array in order to monitor multiple cytokines. Biosensor redeployment can enable chronic monitoring from months to years. The tattooed biosensor array of the present invention includes endogenous reporter cells, naturally tuned to each patient's own biology and can be used to reliably measure the state of a patient in real-time.

Claims17

Dependent claims

A method of monitoring gene expression in a patient comprising:

injecting at least a first nucleic acid at a site in the skin of a patient with a microneedle array produce a biosensor tattoo, the microneedle array comprising:
a backing;
and
one or more microneedles attached to a side of the backing and comprising the first nucleic acid comprising a first gene encoding a colorimetric protein under transcriptional control of a vertebrate transcription factor-responsive element (TRE) such that when transfected into a vertebrate cell, the first gene is expressed differently in the presence of a vertebrate transcription factor that binds the TRE than in the absence of the transcription factor, and the difference in expression of the first gene is optically detectable;
and
detecting an expression level of at least the first gene by detecting a color intensity change at one or more addressable locations in the biosensor tattoo due to expression of the colorimetric protein of at least the first gene.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/568,327Filed 22 Apr 2016 · us provisional application
Priority: 23 Apr 2015
Published: US 2018/0119077 A13 May 2018
Granted: 19 Jan 2021
Term ends: Not estimatedThe source records don’t support an estimate.

Classification13 codes

A61M 37/0015
Other apparatus for introducing media into the body ; Percutany, i.e. introducing medicines into the body by diffusion through the skin: by using…
C12Q 1/68
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
A61K 49/0006
Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for…: skin…
A61K 49/0045
Characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules: the fluorescent agent being a peptide or protein used…