Functional hydrated hyaluronic acid and method for producing coated lactic acid bacteria having excellent intestinal mucoadhesive ability and selective antagonistic action using same

A method for preparing a quadruply coated lactic acid bacteria, the method comprising:

• (a) conducting a primary coating by mixing a water-soluble polymer with a lactic acid bacteria;
• (b) conducting a secondary coating by mixing a functional hydrated hyaluronic acid complexed with a fermented product of a lactic acid bacteria with the primarily coated lactic acid bacteria of step (a), wherein the fermented product of a lactic acid bacteria comprises an adhesion inhibitor of a harmful bacteria, wherein the adhesion inhibitor is selected from the croup consisting of lipoteichoic acid and peptidoglycan, and wherein the functional hydrated hyaluronic acid complexed with the fermented product of a lactic acid bacteria is prepared by a process comprising:
• adding hyaluronic acid to a tyndallized culture medium of a lactic acid bacteria in a ratio of 0.001 to 1 part by weight of hyaluronic acid to 100 parts by weight of a tyndallized culture medium of a lactic acid bacteria, and dissolving the hyaluronic acid in the culture medium of a lactic acid bacteria upon stirring, followed by concentration;
• (c) conducting a tertiary coating by mixing a coating agent having porous particles with the secondarily coated lactic acid bacteria of step (b);
• and
• (d) conducting a quaternary coating by mixing a protein with the tertiarily coated lactic acid bacteria of step (c).