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US 10,894,080 B2Granted
Transgenic VERO-CD4/CCR5 cell line
OwnerChristopher L. Parks, Wayne C. Koffinventors
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Abstract
The present relation relates to a transgenic Vero cell line expressing CD4 and CCR5. The present invention encompasses the preparation and purification of immunogenic compositions which are formulated into the vaccines of the present invention.
Claims8
A Vero cell line transformed with a C—C chemokine receptor type 5 (CCR5 receptor) and differentiation 4 (CD4) receptor polyprotein and expressing a cluster of CD4 receptor and CCR5 receptor,
- wherein the cell line further comprises a recombinant vesicular stomatitis virus (VSV) vector wherein a gene encoding the VSV surface glycoprotein G (VSV G) is functionally replaced by functional clade A HIV Env BG505 (VSVΔG-Env.BG505),
- wherein the cell line is transfected with a vector containing and expressing a nucleic acid sequence encoding an amino acid sequence of an Env.BG505 immunogen encoded by the VSVΔG-Env.BG505,
- wherein the vector comprises the sequence of a VSVΔG-Env.BG505 genomic clone;
- and
- wherein the sequence of the VSVΔG-Env.BG505 genomic clone is SEQ ID NO:
- 1.
3 more elements
Dependencies are inferred from the claim text.