Chromatography based purification strategies for viruses

A method for purifying recombinant infectious virus particles, comprising:

• (i) providing at least one clarified virus sample comprising at least one recombinant infectious virus particle, wherein the at least one recombinant infectious virus particle comprises a measles virus scaffold and is obtained from at least one host cell infected with a virus stock comprising the at least one recombinant infectious virus particle, and wherein the clarification has been performed by a filtration method and the at least one virus sample is treated with a DNAse before or after clarification;
• (ii) purifying the at least one recombinant infectious virus particle by means of chromatography performed using a stationary phase, wherein the stationary phase has a monolithic arrangement and has a pore size of at least 5 μm, at least 6 μm, or at least 7 μm, wherein the mode of adsorption is hydrophobic interaction;
• and
• (iii) directly obtaining purified recombinant infectious virus particles within at least one fraction from the chromatography of step (ii), wherein the purified infectious virus particles contain less than 33.33 ng/mL of contaminating host cell DNA with respect to 1 mL of the at least one fraction.