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US 10,893,928 B2Granted
Decellularized biologically-engineered tubular grafts
AssigneeRegents of the University of Minnesota
InventorsRobert Tranquillo, Zeeshan Syedain, Lee Meier
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Abstract
This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.
Claims23
A decellularized, biologically-engineered graft, made by a method comprising:
- combining matrix-producing cells, fibrinogen and thrombin to produce a cell-seeded fibrin gel,
- shaping and conditioning the gel in the presence of culture medium to result in a cell-produced extracellular matrix comprising circumferentially aligned fibers, wherein the conditioning comprises stretching or distending the gel and/or the extracellular matrix, during which and axial length of the gel and/or the extracellular matrix shortens;
- and
- decellularizing the extracellular matrix to produce the decellularized, biologically engineered graft.
1 more element
A decellularized, biologically-engineered tissue, made by a method comprising:
- combining fibrinogen or fibrinogen-like material, thrombin, and matrix-producing cells to produce a cell-seeded gel composition;
- conditioning the gel composition in the presence of culture medium to remodel the composition into a cell-produced extracellular matrix comprising circumferentially aligned fibers, wherein the conditioning comprises stretching or distending the gel composition and/or the extracellular matrix, during which and axial length of the gel composition and/or the extracellular matrix shortens;
- and
- decellularizing the extracellular matrix to produce a decellularized tissue.
1 more element
Dependencies are inferred from the claim text.