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US 10,890,559 B2Granted
Method and device for accelerated surface-based reactions
AssigneeTechnion Research & Development Foundation Limited
InventorsMoran Bercovici, Merav Karsenty
Life of this patent
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Abstract
An ITP-based system and a method are provided. ITP is used to focus a sample of interest and deliver a high concentration target to a pre-functionalized surface comprising immobilized probes, thus enabling rapid reaction at the sensor site.
Claims10
A method of accelerating a surface-based reaction, the method comprising the following consecutive steps:
- (a) providing a microfluidic channel comprising at least one reaction surface comprising a groove, said groove comprising one or more probes immobilized thereto;
- (b) providing a sample comprising an analyte and focusing said analyte by isotachophoresis (ITP) at an ITP interface, thereby obtaining a focused analyte;
- (c) delivering said focused analyte to said one or more probes immobilized to said groove for hybridization by electro-migrating the ITP interface downstream and obtaining a hybridized analyte and an unhybridized analyte;
- and washing out consisting of hybridized analyte immobilized at the reaction surface and electro-migrating of the ITP interface further downstream from said reaction surface, carrying along said unhybridized analyte, a contaminant, or both;
- thereby accelerating a surface-based reaction.
2 more elements
Dependencies are inferred from the claim text.