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US 10,889,843 B2Granted
Corynebacterium sp. microorganisms having l-lysine-producing ability and l-lysine producing method using same
AssigneeCj Cheiljedang Corporation
InventorsHyo Jeong Byun, Yoon Hee Chung, Hyung Joon Kim, Sun Young Lee, Hyun Koo Nam, Sun Mi Park, Sang Mok Lee
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Abstract
The present invention relates to an L-lysine-producing microorganism of the genus Corynebacterium and a method for producing L-lysine using the same.
Claims6
A modified microorganism of the genus Corynebacterium, wherein a protein comprising the amino acid sequence of SEQ ID NO:
- 1 is inactivated;
- and having enhanced expression of one or more genes selected from the group consisting of aspB (aspartate aminotransferase-encoding gene) lysC (aspartate kinase-encoding gene), asd (aspartate semialdehyde dehydrogenase-encoding gene), dapA (dihydrodipicolinate synthase-encoding gene), dapB (dihydrodipicolinate reductase-encoding gene) and lysA (diaminodipimelate decarboxylase-encoding gene).
A method for producing L-lysine, comprising the step of:
- culturing a microorganism of the genus Corynebacterium in a medium, wherein a protein comprising the amino acid sequence of SEQ ID NO:
- 1 is inactivated, and having enhanced expression of one or more genes selected from the group consisting of aspB (aspartate aminotransferase-encoding gene), lysC (aspartate kinase-encoding gene), asd (aspartate semialdehyde dehydrogenase-encoding gene), dapA (dihydrodipicolinate synthase-encoding gene), dapB (dihydrodipicolinate reductase-encoding gene) and lysA (diaminodipimelate decarboxylase-encoding gene);
- and recovering L-lysine from the microorganism or the culture medium.
A method for producing L-lysine, comprising the step of:
- culturing a microorganism of the genus Corynebacterium in a medium, wherein a gene having the nucleotide sequence of SEQ ID NO:
- 2 is inactivated, and having enhanced expression of one or more genes selected from the group consisting of aspB (aspartate aminotransferase-encoding gene), lysC (aspartate kinase-encoding gene), asd (aspartate semialdehyde dehydrogenase-encoding gene), dapA (dihydrodipicolinate synthase-encoding gene), dapB (dihydrodipicolinate reductase-encoding gene,) and lysA (diaminodipimelate decarboxylase-encoding gene);
- and
- recovering L-lysine from the microorganism or the culture medium.
1 more element
A method for producing L-lysine, comprising the step of:
- culturing a microorganism of the species Corynebacterium glutamicum in a medium, wherein a protein comprising the amino acid sequence of SEQ ID NO:
- 1 is inactivated, and having enhanced expression of one or more genes selected from the group consisting of aspB (aspartate aminotransferase-encoding gene), lysC (aspartate kinase-encoding gene), asd (aspartate semialdehyde dehydrogenase-encoding gene), dapA (dihydrodipicolinate synthase-encoding gene), dapB (dihydrodipicolinate reductase-encoding gene) and lysA diaminodipimelate decarboxylase-encoding gene);
- and
- recovering L-lysine from the microorganism or the culture medium.
1 more element
Dependencies are inferred from the claim text.