Conversion of methylglyoxal into hydroxyacetone using novel enzymes and applications thereof
AssigneeMetabolic Explorer
InventorsLaetitia Faure, Gwenaëlle Corre, Céline Raynaud, Laurence Dumon-Seignovert
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Abstract
The present invention relates to new methylglyoxal reductase (MGR) enzymes which are useful for efficiently converting methylglyoxal into hydroxyacetone. The invention more particularly relates to a method for efficiently converting methylglyoxal into hydroxyacetone using said enzymes, to a method for producing 1,2-propanediol using a microorganism overexpressing said enzymes, and to said microorganism.
Claims19
A method for the fermentative conversion of methylglyoxal into hydroxyacetone, comprising the step of expressing, in a microorganism, at least one methylglyoxal reductase having a catalytic efficiency kcat/Km equal or superior to 5 mM−1s−1 wherein said methylglyoxal reductase is selected from the group consisting of YjgB having the sequence of SEQ ID NO:
- 1, YjgB* (N240Y) having the sequence of SEQ ID NO:
- 9, YigB*(I165V) having the sequence of SEQ ID NO:
- 125 and YigB*(Q39R/I165V/A296V) having the sequence of SEQ ID NO:
- 127.
Dependencies are inferred from the claim text.