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US 10,889,821 B2Granted
Organic acid synthesis from C1 substrates
AssigneeAlliance for Sustainable Energy, LLC
InventorsCalvin A. Henard, Michael T. Guarnieri
Life of this patent
Estimating the term…
Abstract
Presented herein are biocatalysts and methods for converting C1-containing materials to organic acids such as muconic acid or adipic acid.
Claims12
An engineered cell, comprising an exogenously added gene encoding a 3-dehydroshikimate dehydratase (AroZ), a protocatechuic acid decarboxylase (AroY), a catechol 1,2-dioxygenase (CatA), a phospho-2-dehydro-3-deoxyheptonate aldolase (AroG), an anthranilate synthase (TrpE), a phosphoenolpyruvate synthase, or a transketolase;
- wherein the engineered cell is a methanotroph and is able to convert a C1 substrate to a muconic acid and the cell comprises genes encoding the 3-dehydroshikimate dehydratase (AroZ), the protocatechuic acid decarboxylase (AroY), and the catechol 1,2-dioxygenase (CatA).
Dependencies are inferred from the claim text.