US 10,889,806 B2 · Engineered pantothenate kinase variant enzymes

Abstract

The present invention provides engineered pantothenate kinase (PanK) enzymes, polypeptides having PanK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PanK enzymes are also provided. The present invention further provides compositions comprising the PanK enzymes and methods of using the engineered PanK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Claims29

Dependent claims

An engineered pantothenate kinase polypeptide comprising an amino acid sequence having at least 85% sequence identity to SEQ ID NO:

2, or to a functional fragment thereof, wherein said polypeptide or fragment thereof comprises an amino acid substitution corresponding to amino acid position 277 and/or 281 in SEQ ID NO:
2, wherein said polypeptide has increased ability to convert ethynyl glyceraldehyde to ethynyl glyceraldehyde phosphate when compared the wild-type polypeptide having the amino acid sequence SEQ ID NO:
2.

Dependencies are inferred from the claim text.

Facts

Application

16/460,147Filed 2 Jul 2019 · us provisional application

Priority

9 Jul 2018

Published

US 2020/0010815 A19 Jan 2020

Granted

12 Jan 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 9/1205
Transferring phosphorus containing groups, e.g. kinases: phosphotransferases with an alcohol group as acceptor , e.g. protein kinases
C12Y 207/01033
Phosphotransferases with an alcohol group as acceptor: pantothenate kinase
C12N
Microorganisms or enzymes
C12Y
Enzymes

Engineered pantothenate kinase variant enzymes

Life of this patent

Engineered pantothenate kinase variant enzymes

Life of this patent

Abstract

Claims29