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US 10,889,802 B2Granted
B-cell cultivation method
AssigneeHoffmann-La Roche Inc.
InventorsSonja Offner, Friederike Jung
Life of this patent
Estimating the term…
Abstract
Herein is reported a method for co-cultivating one or more B-cells comprising the step of incubating the one or more B-cells with EL4-B5 cells, whereby the EL4-B5 cells have been obtained/are from a cultivation of EL4-B5 cells that has a cell density of from 600,000 cells/ml up to 1,500,000 cells/ml.
Claims15
A method for co-cultivating one or more B-cells comprising the steps of
- cultivating EL4-B5 cells to a cell density of more than 1,000,000 cells/ml to 1,500,000 cells/ml, and
- incubating the one or more B-cells with an aliquot of the EL4-B5 cells obtained in the previous step.
Dependencies are inferred from the claim text.