Methods and products for in vivo enzyme profiling
AssigneeMassachusetts Institute of Technology
InventorsSangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
Claims30
A reagent comprising
- a carrier domain, wherein the carrier domain is a particle and is greater than 5 nm in size;
- an enzyme susceptible domain linked to the carrier domain;
- and,
- a signature molecule linked to the enzyme susceptible domain, wherein the signature molecule comprises a peptide or nucleic acid and is optimized for LC/MS detection.
An implantable device housing a structure comprising a carrier domain linked to a signature producing agent that is capable of modifying a biological substrate to produce a signature molecule.
A reagent comprising
- a carrier domain having a plurality of enzyme susceptible domains linked to the carrier domain wherein each enzyme susceptible domain is linked to a non-fluorescent signature molecule.
A composition comprising
- a plurality of pro-diagnostic reagents comprising a carrier domain, an enzyme susceptible domain linked to the carrier domain;
- and, a non-fluorescent signature molecule linked to the enzyme susceptible domain.
Dependencies are inferred from the claim text.