US 10,883,990 B2 · Methods and kits for determining tuberculosis infection status

Abstract

There is provided methods of determining tuberculosis (TB) infection status in an individual comprising: (i) providing a sample comprising T-cells; (ii) exposing the sample of (i) to one or more TB antigens; (iii) identifying T-cells in the sample that are CD4 positive and (a) secrete TNF-α without secreting IFN-γ; or (b) secrete IFN-γ without secreting TNF-α; (iv) identifying those cells of (iii) which are also CCR7 and, CD127 negative; and optionally (v) calculating the cells identified in (iv) as a percentage of those identified in (iii); wherein the identification of cells in (iv) and/or the percentage of T-cells calculated in (v) correlates to TB infection status of the individual, and wherein steps (iii) and (iv) can be carried out either sequentially or simultaneously. There are also provided compositions and kits for use in such methods.

Claims11

Dependent claims

A method of determining tuberculosis (TB) infection status in an individual comprising:

(i) providing a sample comprising T-cells from said individual;
(ii) exposing the sample of (i) to one or more TB antigens;
(iii) identifying T-cells in the sample that are CD4 positive and (a) secrete TNF-α without secreting IFN-γ;
or (b) secrete IFN-γ without secreting TNF-α;
(iv) assessing the number of CD4+ T cells of (iii)(a) or (b) for CCR7 and CD127 expression;
and
(v) calculating the number of CD4+, CCR7− and CD127− cells identified in (iv) as a percentage of the CD4+ T cells identified in (iii)(a) or (b);
the higher the percentage of CD4+ cells that are CCR7− and CD127− calculated in step (v), the greater the likelihood that said individual has an active TB infection, and wherein steps (iii) and (iv) can be carried out either sequentially or simultaneously.

5 more elements

Dependencies are inferred from the claim text.

Methods and kits for determining tuberculosis infection status

Life of this patent

Methods and kits for determining tuberculosis infection status

Life of this patent

Abstract

Claims11