Peptides of M. tuberculosis for a screening test for HIV positive patients at high-risk for tuberculosis
AssigneeNew York University
InventorSuman Laal
Life of this patent
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Abstract
Provided are peptides suitable for early detection of active M. tuberculosis (Mtb) infection in immunocompromised individuals. The peptides can form complexes with antibodies directed to Mtb antigens MS, MPT51, ESAT6 or CFPIO. Also provided are methods for detected of complexes of the peptides and the antibodies. The presence of complexes aids in predicting risk in immunocompromised individuals of developing active tuberculosis.
Claims18
A method for early detection of active M. tuberculosis (Mtb) infection in an individual comprising:
- contacting a biological sample obtained from the individual with a peptide comprising the sequence as set forth in SEQ ID NOs 1-18, 20, or 27-29, wherein the peptide is 15-20 amino acids long;
- and detecting the formation of a complex of the peptide with antibodies present in the sample, wherein formation of the complex is an indication of active Mtb infection in the individual.
A method for predicting risk of an asymptomatic immunocompromised individual developing active tuberculosis (TB) comprising:
- a) contacting a biological sample obtained from the individual with a peptide comprising the sequence as set forth in SEQ ID NOs 1 18, 20, or 27-29, wherein the peptide is 15-20 amino acids long;
- b) detecting the presence of a complex formed of the peptide with the an antibody in the sample, wherein the antibody is specific for an Mtb antigen selected from the group consisting of malate synthase (MS), MPT51, ESAT6 and CFP10;
- c) comparing the level of complexes in the sample to the level of complexes in a control;
- and
- d) identifying the individual to be at risk of developing active TB if the level of complexes in the sample from the individual is more than the level of complexes in the control.
A method of monitoring the progression toward active tuberculosis (TB) in an immunocompromised individual comprising:
- a) contacting each of at least two biological samples obtained from the individual at two different times with a peptide comprising the sequence as set forth in SEQ ID NOs 1 18, 20, or 27-29, wherein the peptide is 15-20 amino acids long;
- b) detecting the formation of a complex of the peptide with an antibody present in the sample, wherein the antibody is specific for malate synthase (MS), MPT51, ESAT6, or CFP10;
- c) comparing the level of the complexes in the two samples;
- and
- d) if the level of the complexes is more in the sample obtained at the later time, identifying the individual as progressing toward active TB.
Dependencies are inferred from the claim text.