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US 10,883,988 B2Granted
Method and kit for immunological detection of Mycobacterium tuberculosis complex
AssigneeTauns Co., Ltd.
InventorsUrao Nonaka, Toshiyuki Kitagawa
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AssigneeTauns Co., Ltd.
InventorsUrao Nonaka, Toshiyuki Kitagawa
Estimating the term…
Definitive diagnosis and early start of treatment cannot be made for tuberculosis since conventional methods for detecting a Mycobacterium tuberculosis complex require a long time plus enormous labor and expense. Because detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of the Mycobacterium tuberculosis complex-specific secretory protein, there is a risk infection with the Mycobacterium tuberculosis complex will be missed. The present method and a kit address these long-standing needs by more rapidly and conveniently detecting a Mycobacterium tuberculosis complex, without culturing a biological sample containing the Mycobacterium tuberculosis complex, in which a biological sample is subjected to a heat-treatment so as to extracellularly secrete a Mycobacterium tuberculosis complex-specific secretory protein, such as at least one of MPB70, ESAT-6, or CFP-10 and subjecting the resulting treated sample to an immunological measurement/assay.
A method for detecting the presence of a Mycobacterium tuberculosis complex-specific protein in a heat-treated biological sample, the method comprising
A kit for detection of the presence of a Mycobacterium tuberculosis complex-specific protein selected from the group consisting of MPB70, ESAT-6, and CFP-10, in a heat-treated biological sample comprising at least
A method for promoting extracellularly secreting a Mycobacterium tuberculosis complex-specific protein, comprising subjecting a biological sample containing the Mycobacterium tuberculosis complex to a heat-treatment at 40° C. to 60° C. for 15 to 60 minutes whereby said Mycobacterium tuberculosis complex-specific protein is extracellularly secreted and is at least one protein selected from the group consisting of MPB70, ESAT-6, and CFP-10.
A kit for detecting the presence of a Mycobacterium tuberculosis complex-specific protein selected from the group consisting of MPB70, ESAT-6, and CFP-10 in a heat-treated biological sample, comprising at least a treatment container in which a biological sample to be tested for the Mycobacterium tuberculosis complex-specific protein is subjected to a heat-treatment, wherein the treatment container is adapted to allow the biological sample to be heated at 40° C. to 60° C. for a time sufficient for Mycobacterium tuberculosis complex, if present in the biological sample, to extracellularly secrete the at least one Mycobacterium tuberculosis complex-specific protein selected from the group consisting of MPB70, ESAT-6, and CFP-10, said treatment container having an opening comprising a dropper nozzle and a filter for removing solid matter and discharging drops of heat-treated and filtered biological sample;
A method for detecting the presence of a Mycobacterium tuberculosis complex-specific protein in a heat-treated biological sample as an indicator for the presence of Mycobacterium tuberculosis complex in the biological sample, the method comprising
Dependencies are inferred from the claim text.