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US 10,883,978 B2Granted
Method and device for calibration of biological flux
AssigneeAgilent Technologies, Inc.
InventorAndrew C. Neilson
Life of this patent
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Abstract
An apparatus for calibrating a flux analyzer comprises a first frame; a second frame; and a permeable membrane. The first frame and the second frame are connected or integrally formed. A method for calibrating a flux analyzer is provided which uses an artificial standard rather than a biological standard.
Claims19
An apparatus for calibrating a flux analyzer comprising:
- a first frame comprising a plurality of wells, each well having a well opening at its bottom;
- a second frame connected or integrally formed with the first frame wherein the second frame defines a chamber and an inlet to the chamber, and the second frame has at least one chamber opening, wherein the chamber opening at least partially overlaps the well openings;
- and
- a selectively permeable membrane between the first and second frames that separates the well openings from the chamber opening, wherein the membrane is substantially impermeable to water;
- wherein the second frame comprises a removable or slidable wall opposite the first frame and adapted to allow access to the chamber.
2 more elements
A method of calibrating a flux analyzer comprising:
- filling the plurality of wells of an apparatus with a test solution, wherein the test solution contains a first analyte having a known value, and the apparatus comprises:
- a first frame comprising a plurality of wells, each well having a well opening at its bottom;
- a second frame connected or integrally formed with the first frame wherein the second frame defines a chamber and an inlet to the chamber, and the second frame has at least one chamber opening, wherein the chamber opening at least partially overlaps the well openings;
- and
- a selectively permeable membrane between the first and second frames that separates the well openings from the chamber opening;
- contacting the test solution with a flux sensor that measures the first analyte;
- supplying a test fluid to the chamber of the second frame of the apparatus, wherein the test fluid contains a substance that crosses the membrane;
- measuring flux of the first analyte to obtain a measured flux.
5 more elements
A method of making an apparatus for calibrating a flux analyzer, the method comprising:
- providing a membrane, a first frame and a second frame;
- a first frame comprising a plurality of wells, each well having a well opening at its bottom;
- a second frame that defines a chamber and an inlet to the chamber, and the second frame has a chamber opening;
- and
- a selectively permeable membrane, wherein the membrane is substantially impermeable to water;
- aligning the well openings to at least partially overlap the chamber opening;
- placing the membrane between the first and second frames to separate the well openings from the frame opening;
- fastening the membrane between the first and second frames;
- wherein the step of providing a membrane comprises:
- determining a desired flux rate of a first analyte through the membrane;
- selecting membrane parameters to obtain the desired flux rate of the first analyte across the permeable membrane;
- and
- selecting a membrane having the membrane parameters to provide the desired flux rate.
10 more elements
Dependencies are inferred from the claim text.