US 10,883,963 B2 · Electrochemical measurement using phenylenediamine derivative

Abstract

The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), R1 is either H or an alkyl group (CmH2m+1), m is an integer of 1-4, R2 is an alkyl group (CnH2n+1), and n is an integer of 1-4.)

Claims5

Dependent claims

A method of measuring an endotoxin and/or (1→3)-β-D-glucan in a sample, comprising:

(1) contacting the sample with a measuring substrate represented by formula (2):
wherein R1 is H, R2 is methyl, and X1 is a peptide with an Arg (R) residue at the C-terminus which is coupled to the NH group of the measuring substrate by an amide bond and an enzyme that degrades the measuring substrate when the endotoxin and/or (1→3)-β-D-glucan is present in the sample, and
(2) measuring the cleavage of the peptide from the measuring substrate by the enzyme by an electrochemical measurement method.

Dependencies are inferred from the claim text.

Facts

Application

16/076,614Filed 15 Feb 2017

Priority

JP 2016-02692516 Feb 2016

Published

US 2019/0049408 A114 Feb 2019

Granted

5 Jan 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

G01N 27/48
Systems: using polarography, i.e. measuring changes in current under a slowly-varying voltage
C07C 211/51
With at least two amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton: phenylenediamines
C07K 9/00
Peptides having up to 20 amino acids, containing saccharide radicals and having
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria

Electrochemical measurement using phenylenediamine derivative

Life of this patent

Electrochemical measurement using phenylenediamine derivative

Life of this patent

Abstract

Claims5