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US 10,883,957 B2Granted
Magnesium sensing membrane for potentiometric ion selective electrode for measuring ionized magnesium and methods of production and use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsWei Zhang, Kevin Horan
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Abstract
A magnesium sensing membrane is disclosed for use in a potentiometric ion selective electrode that exhibits improved stability upon exposure to surfactant-containing reagents. Kits containing same are disclosed, along with methods of production and use of the magnesium sensing membrane.
Claims14
A potentiometric ion selective electrode that detects ionized magnesium in a biological sample, the potentiometric ion selective electrode comprising:
- a magnesium sensing membrane that detects ionized magnesium in a biological sample, the membrane comprising:
- an ionophore having a tripodal stereochemical structure;
- a lipophilic borate salt, wherein the lipophilic borate salt is present in an amount that provides a mol ratio of lipophilic borate salt to ionophore in a range of from about 75 mol % to about 100 mol %;
- and
- a polymer matrix in which the ionophore and lipophilic borate salt are disposed, wherein the polymer matrix comprises a polymer and a plasticizer, wherein the polymer is selected from the group consisting of poly(vinyl chloride), polyurethane, and combinations thereof;
- wherein the potentiometric ion selective electrode is more selective to Mg2+ than to Ca2+;
- and
- wherein the potentiometric ion selective electrode is substantially stable upon exposure to a reagent comprising a poly(ethylene oxide) surfactant.
5 more elements
A method, comprising the steps of:
- contacting a potentiometric ion selective electrode with a biological sample, wherein the potentiometric ion selective electrode comprises a magnesium sensing membrane that detects ionized magnesium in a biological sample, and wherein the membrane comprises:
- (i) an ionophore having a tripodal stereochemical structure;
- (ii) a lipophilic borate salt present in an amount that provides a mol ratio of lipophilic borate salt to ionophore in a range of from about 75 mol % to about 100 mol %;
- and (iii) a polymer matrix in which the ionophore and lipophilic borate salt are disposed, wherein the polymer matrix comprises a polymer and a plasticizer, wherein the polymer is selected from the group consisting of poly(vinyl chloride), polyurethane, and combinations thereof;
- and wherein the potentiometric ion selective electrode is more selective to Mg2+ than to Ca2+, and wherein the potentiometric ion selective electrode is substantially stable upon exposure to a reagent comprising a poly(ethylene oxide) surfactant;
- and
- measuring a level of magnesium ion in the biological sample using the potentiometric ion selective electrode.
4 more elements
A kit comprising:
- a potentiometric ion selective electrode comprising a magnesium sensing membrane that detects ionized magnesium in a biological sample, wherein the membrane comprises:
- (i) an ionophore having a tripodal stereochemical structure;
- (ii) a lipophilic borate salt present in an amount that provides a mol ratio of lipophilic borate salt to ionophore in a range of from about 75 mol % to about 100 mol %;
- and (iii) a polymer matrix in which the ionophore and lipophilic borate salt are disposed, wherein the polymer matrix comprises a polymer and a plasticizer, wherein the polymer is selected from the group consisting of poly(vinyl chloride), polyurethane, and combinations thereof;
- and wherein the potentiometric ion selective electrode is more selective to Mg2+ than to Ca2+, and wherein the potentiometric ion selective electrode is substantially stable upon exposure to a reagent comprising a poly(ethylene oxide) surfactant;
- and
- at least one reagent comprising a poly(ethylene oxide) surfactant, wherein the poly(ethylene oxide) surfactant is represented by the structure of Formula V, VI, or VII:
- wherein n in Formula V is in the range of from 9 to 10, and n in Formula VII is 100.
5 more elements
Dependencies are inferred from the claim text.