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US 10,883,903 B2Granted
Method for rendering biological material transparent and processing kit for rendering biological material transparent
AssigneeRiken
InventorsTakeshi Imai, Meng-Tsen Ke
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Abstract
A method according to the present invention for making a biological material transparent, includes the step of: making the biological material transparent by causing a solution containing fructose to permeate into the biological material, and a kit according to the present invention for a clearing treatment for making a biological material transparent, includes a solution containing fructose.
Claims8
A method for making a biological material transparent, comprising the step of:
- (a) treating the biological material with a solution containing fructose and causing the solution to permeate into the biological material, thereby making the biological material transparent,
- wherein the solution contains the fructose at a concentration of 50% (w/v) to 200% (w/v),
- wherein the treating is carried out in a container for a clearing treatment,
- wherein the biological material is (i) a tissue or an organ derived from a multicellular animal or (ii) a whole of a multicellular animal which is not a human,
- wherein the tissue or the organ or the whole of a multicellular animal which is not a human contains a fluorescent protein or a fluorescent chemical substance, or is injected with the fluorescent chemical substance after the step (a).
2 more elements
Dependencies are inferred from the claim text.