Fluorescence measurement of samples

A method of determining the fluorescence of a sample, the method comprising:

• illuminating a calibration reference under one or more narrow-band light sources, each of the one or more narrow-band light sources configured to emit light that contains power in an excitation band, but not in a fluorescence band, of the sample;
• capturing, with a light sensing device, illumination measurement data of the calibration reference under the one or more narrow-band light sources;
• illuminating the sample under analysis under each of the one or more narrow-band light sources;
• capturing, with the light sensing device, sample measurement data of light from each of the one or more narrow-band light sources that has illuminated the sample, wherein the sample measurement data includes at least an excitation response curve, where the excitation response curve includes the sample measurement data and the-illumination measurement data, and a fluorescent response curve under each of one or more narrow-band light sources;
• generating a fluorescent profile value using the fluorescent response curve of the measurement data, by obtaining the area under the fluorescence response curve divided by the area under the excitation response curve;
• calculating an excitation profile value using the excitation response curve and the fluorescent response curve;
• and
• outputting both the fluorescent profile and the excitation profile.