Aptamers with binding affinity to norovirus
AssigneeNorth Carolina State University
InventorsLee-Ann Jaykus, Blanca Irene Escudero-Abarca, Matthew D. Moore, Helen Rawsthorne
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Abstract
The instant disclosure provides norovirus-binding aptamers, compositions comprising such aptamers, and methods of using and producing such aptamers. The aptamers are useful, for example, for detecting the presence of norovirus in test samples, for capturing and/or concentrating norovirus from test samples, for evaluating the efficacy of therapeutic agents in patients diagnosed with a norovirus infection, and for evaluating the efficacy of norovirus vaccines.
Claims15
A method of detecting the presence of at least one norovirus strain in a test sample, the method comprising:
- (a) contacting said test sample with a norovirus-binding aptamer comprising:
- (i) a norovirus-binding motif wherein said norovirus-binding motif comprises one or more of SEQ ID NOS:
- 113, 114, 116-118, 120, or 101-106;
- and/or
- (ii) the nucleic acid sequence of SEQ ID NO:
- 27, 30, 31, 35, or 39, or variants thereof having at least 90% sequence identity and having norovirus-binding activity;
- and
- (b) detecting the presence of said norovirus-binding aptamer bound to norovirus in said test sample, wherein detection of bound aptamer indicates the presence of at least one norovirus strain.
Dependencies are inferred from the claim text.