Method and system of nanopore-based information encoding
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Mirkó Palla, Peter Benjamin Stranges, Jeffrey Matthew Nivala
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Abstract
This invention provides methods and systems of DNA synthesis including providing an encoding unit comprising an enzyme, a single-stranded DNA (ssDNA) and a nanopore, providing a lipid bilayer having on opposite sides a cis and a trans reservoir each having a different buffer composition, wherein the nanopore is within the lipid bilayer and the enzyme and the ssDNA are in the cis reservoir, providing an electrode over the lipid bilayer wherein the electrode can modulate voltage across the lipid bilayer, wherein the enzyme catalyzes DNA synthesis in response to the voltage.
Claims22
A method of encoding information through synthesis of a DNA strand comprising
- providing an encoding unit comprising a DNA polymerase, a single-stranded DNA (ssDNA) and a nanopore, wherein the DNA polymerase is conjugated to the nanopore,
- providing a lipid bilayer having on opposite sides a cis and a trans reservoir each having a different buffer composition, wherein the nanopore is within the lipid bilayer and the DNA polymerase and the ssDNA are in the cis reservoir,
- introducing into the cis reservoir a specific nucleotide corresponding to an encoding scheme for information storage in synthesized DNA, and
- applying a voltage across the lipid bilayer via an electrode wherein the electrode can modulate the voltage across the lipid bilayer,
- wherein catalytic ions Mg2+ or Co2+ are injected to the cis side from the trans side, thereby activating the DNA polymerase to catalyze addition of the specific nucleotide to the single stranded DNA during DNA synthesis, and
- repeating catalytic addition to create synthesized DNA wherein the information is encoded and stored in the synthesized DNA.
A method of DNA synthesis comprising applying a voltage across a lipid bilayer having one or more encoding units associated therewith, wherein each encoding unit comprises a DNA polymerase an enzyme, a single-stranded DNA (ssDNA) and a nanopore, wherein the lipid bilayer has on opposite sides a cis and a trans reservoir each having a different buffer composition, wherein the trans reservoir comprises catalytic ions, wherein the nanopore is within the lipid bilayer and the DNA polymerase enzyme and the ssDNA are conjugated to the nanopore and are in the cis reservoir, and wherein the catalytic ions move from the trans reservoir to the cis reservoir in response to the voltage and the DNA polymerase enzyme is activated and catalyzes DNA synthesis in the presence of a nucleotide response to the voltage.
Dependencies are inferred from the claim text.