Method of isolating biochemical molecules on microarray substrate

A method of isolating biochemical molecules on a microarray substrate, the method comprising:

• providing a microarray substrate to which clusters of different kinds of biochemical molecules being classified by individual spot units are attached, the individual spots being regularly arranged thereon,
• wherein the microarray substrate is one of a substrate including a sacrificial layer, a substrate surface-coated with a sacrificial layer, and combinations thereof;
• obtaining location information of the individual spot in which a desired cluster among clusters of the biochemical molecules locates;
• locating an extraction tool for applying energy to selectively isolate the desired cluster according to the location information;
• and
• selectively isolating the desired cluster from the sacrificial layer of the microarray substrate by applying energy in a contact or non-contact manner using the extraction tool, such that the desired cluster is selectively and directly transferred to a selected well in a reservoir well plate below each of the individual spot units when the desired cluster is severed from the microarray substrate,
• wherein the microarray substrate is irradiated with a pulse laser to apply energy.