Devices, systems and methods for detecting viable infectious agents in a fluid sample

A method for detecting a susceptibility of an infectious agent to an anti-infective, the method comprising:

• introducing a fluid sample to a first surface and a second surface, wherein the fluid sample comprises the infectious agent;
• exposing the first surface comprising the infectious agent to a first solution, wherein the first surface is a filter surface of a first filter, wherein the first filter is within a first filter housing;
• separating the first solution from the infectious agent after the first solution is exposed to the first surface;
• directing the first solution to a first sensor through an opening in the first filter housing after the first solution is exposed to the first surface;
• monitoring a first electrical characteristic of a first sensor upon introducing the first solution to the first sensor, wherein the first sensor comprises a first reference electrode and wherein the first electrical characteristic of the first sensor changes as a result of a cellular activity undertaken by the infectious agent;
• exposing the second surface comprising the infectious agent to a second solution, wherein at least one of the second surface and the second solution comprises the anti-infective, wherein the second surface is separate from the first surface and is a filter surface of a second filter, and wherein the second filter is within a second filter housing;
• separating the second solution from the infectious agent after the second solution is exposed to the second surface;
• directing the second solution to a second sensor through an opening in the second filter housing after the second solution is exposed to the second surface;
• monitoring a second electrical characteristic of a second sensor upon introducing the second solution to the second sensor, wherein the second sensor comprises a second reference electrode and wherein the second electrical characteristic of the second sensor changes as a result of a cellular activity undertaken by the infectious agent;
• and
• comparing the first electrical characteristic and the second electrical characteristic to assess the susceptibility of the infectious agent to the anti-infective.