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US 10,883,130 B2Granted
Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N
AssigneeConagen Inc.
InventorsGuohong Mao, Michael Batten, Phillip Hunt, Oliver (Xiaodan) Yu
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Abstract
The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.
Claims17
A biosynthetic method of preparing rebaudioside N, the method comprising:
- reacting a steviol glycoside composition comprising rebaudioside A with a rhamnose donor moiety in the presence of a first recombinant polypeptide having 1,2-rhamnosytransferase activity to produce rebaudioside J;
- wherein said first recombinant polypeptide comprises the amino acid sequence of SEQ ID NO:
- 3;
- and
- reacting said rebaudioside J with a glucose donor moiety in the presence of a second recombinant polypeptide having glucosyltransferase activity to produce rebaudioside N, wherein said second recombinant polypeptide comprises the amino acid sequence of SEQ ID NO:
- 13.
3 more elements
A biosynthetic method of preparing rebaudioside J, the method comprising:
- reacting a steviol glycoside composition comprising rebaudioside A with a rhamnose donor moiety in the presence of a recombinant polypeptide having 1,2-rhamnosytransferase activity;
- wherein said recombinant polypeptide comprises the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.