Methods and compositions for degrading cellulosic material

An enzyme composition comprising a CEL6 polypeptide having cellobiohydrolase II activity, a GH10 polypeptide having xylanase activity, and one or more cellulolytic enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase, wherein one or both of the CEL6 polypeptide having cellobiohydrolase II activity and the GH10 polypeptide having xylanase activity are foreign to the one or more cellulolytic enzymes;

• wherein the CEL6 polypeptide having cellobiohydrolase II activity is selected from the group consisting of:
• (a) a polypeptide having cellobiohydrolase II activity comprising an amino acid sequence having a sequence identity of at least 90% to amino acids 18 to 482 of SEQ ID NO:
• 30;
• (b) a polypeptide having cellobiohydrolase II activity encoded by a polynucleotide that hybridizes under very high stringency conditions with nucleotides 52 to 1809 of SEQ ID NO:
• 29, or the full-length complement thereof, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• (c) a polypeptide having cellobiohydrolase II activity encoded by a polynucleotide comprising a nucleotide sequence having a sequence identity of at least 90% to nucleotides 52 to 1809 of SEQ ID NO:
• 29;
• and
• (d) a polypeptide having cellobiohydrolase II activity comprising amino acids 18 to 482 of SEQ ID NO:
• 30;
• and
• wherein the GH10 polypeptide having xylanase activity is selected from the group consisting of:
• (a) a polypeptide having xylanase activity comprising an amino acid sequence having a sequence identity of at least 90% to amino acids 20 to 369 of SEQ ID NO:
• 72;
• (b) a polypeptide having xylanase activity encoded by a polynucleotide that hybridizes under very high stringency conditions with nucleotides 58 to 1107 of SEQ ID NO:
• 71, or the full-length complement thereof, wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• (c) a polypeptide having xylanase activity encoded by a polynucleotide comprising a nucleotide sequence having a sequence identity of at least 90% to nucleotides 58 to 1107 of SEQ ID NO:
• 71;
• and
• (d) a polypeptide having xylanase activity comprising amino acids 20 to 369 of SEQ ID NO:
• 72;
• wherein the one or more cellulolytic enzymes comprise a beta-glucosidase, a Trichoderma reesei cellobiohydrolase I (CEL7A), a Trichoderma reesei cellobiohydrolase II (CEL6A), and a Trichoderma reesei endoglucanase I (CEL7B).