Method of producing gentiobiose or glucose from β-glucan using β-1,6-endoglucanase
AssigneeKorea University Research and Business Foundation
InventorsKyoung Heon Kim, Damao Wang, Do Hyoung Kim
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Abstract
The present invention relates to a novel β-1,6-endoglucanase producing gentiobiose or glucose from β-glucan, and more specifically, the present invention provides an effect of producing gentiobiose or glucose at high yield through β-1,6-endoglucanase showing β-1,6-endoglucanase activity on β-glucan.
Claims4
A method of producing gentiobiose or glucose, the method comprising reacting a β-1,6-endoglucanase comprising the amino acid sequence of SEQ ID NO:1 with, as a substrate, one or more selected from the group consisting of laminarin and pustulan to produce gentiobiose and/or glucose as a reaction product,
- wherein the reaction product does not include an oligosaccharide with other degree of polymerization except for gentiobiose and glucose,
- wherein the reacting of the β-1,6-endoglucanase and the substrate is performed at a temperature ranging from 20° C. to 45° C. and a pH of 5 to 10, and for 5 minutes to 1 day.
Dependencies are inferred from the claim text.