Method for producing aldehyde

A method for producing an objective substance, the method comprising:

• producing the objective substance by using a microorganism having an ability to produce the objective substance,
• wherein said producing comprises:
• (A) cultivating cells of the microorganism in a culture broth or medium containing a carbon source to produce and accumulate the objective substance in the culture broth or medium;
• (B) cultivating cells of the microorganism in a culture broth or medium containing a precursor of the objective substance to produce and accumulate the objective substance in the culture broth or medium;
• or
• (C) allowing cells of the microorganism to act on a precursor of the objective substance in a reaction mixture to produce and accumulate the objective substance in the reaction mixture,
• wherein the objective substance is an aldehyde,
• wherein the precursor is a substance selected from the group consisting of protocatechuic acid, vanillic acid, benzoic acid, L-phenylalanine, cinnamic acid, and combinations thereof,
• wherein the cells of the microorganism is a bacterium or yeast,
• wherein the cells of the microorganism have been modified to have an aldehyde oxidoreductase gene, and
• wherein the aldehyde oxidoreductase gene encodes a protein selected from the group consisting of:
• (a) a protein comprising the amino acid sequence of SEQ ID NO:
• 18, 79, or 83;
• (b) a protein comprising the amino acid sequence of SEQ ID NO:
• 18, 79, or 83 but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has aldehyde oxidoreductase activity;
• (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
• 18, 79, or 83, and wherein said protein has aldehyde oxidoreductase activity.