DNA plasmids for the fast generation of homologous recombination vectors for cell line development
AssigneeTemple University of the Commonwealth System of Higher Education
InventorOscar M. Perez-Leal
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Abstract
The present invention provides homologous recombination vectors to insert transgenic DNA in cells. These vectors shorten the production time and allow for easy generation of genetically modified cells. The invention allows the user to test multiple tags and to generate homozygous modified cell line using the homologous recombination vector. The invention can be used to generate knockout cells, to generate cell lines with knockin genes, to generate cell lines for drug screening against any target, to create transgenic animals, or in gene therapy.
Claims1
An HDR donor vector comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
Dependencies are inferred from the claim text.