Adeno-associated virus variants and methods of use thereof

A recombinant adeno-associated virus (rAAV) virion comprising:

• a) a variant AAV capsid protein, wherein the variant AAV capsid protein comprises an amino acid sequence having at least 95% amino acid sequence identity to the sequence set forth in SEQ ID NO:
• 16, wherein the amino acid at position 264 is a T, Q, or A, position 448 is an S or A, position 459 is a T or N, position 470 is an S or A, position 495 is an S or T, position 533 is a D or E, position 547 is a Q, E, or T, position 555 is a T or A, position 557 is an E or D, position 561 is an M, L, or I, position 563 is an S or N, position 593 is an A, Q, or V, position 596 is an A or T, position 661 is an A, E, or T, position 662 is a V, T, or A, position 664 is a T or S, position 718 is an N or S, and position 723 is an S or T;
• and
• b) a heterologous nucleic acid comprising a nucleotide sequence encoding a gene product.