Transduction buffer
AssigneeKoninklijke Nederlandse Akademie van Wetenschappen
InventorsNiels Geijsen, Diego Sebastián D'Astolfo
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Abstract
The invention relates to transduction compounds, buffers and methods for introducing molecules into cells. The invention also relates to methods of treatment, pharmaceutical compositions and other uses of the transduction compounds and buffers. The invention also relates to modified cells obtainable by the transduction compounds, buffers and methods of the invention.
Claims21
An in vitro method for transducing a molecule of interest into a cell,
- wherein the method comprises contacting said cell with the molecule of interest and a transduction buffer thereby transducing the molecule of interest into the cell, wherein the molecule of interest comprises a protein, wherein the transduction buffer comprises
- (i) a transduction compound,
- (ii) a salt selected from a sodium, lithium, potassium, caesium or rubidium salt, or an activator/enhancer of a sodium-hydrogen transporter;
- wherein the transduction compound is selected from compounds 42, 31, 45, 43, 34, 41, 40, 39, 33, 44, 22, 20, 03, 30, 15, 38, 35, 11, 10, 28, 37, 01, 29, 02, 21, 36, 26, 16, 04, 19, 23, 24, 12, 14, 13, 05, 08 and 27 in Table 1;
- and wherein the transduction buffer has an osmolality of at least 750 mOsm/kg and a pH of between about 6 and about 8.
A method for modifying a nucleic acid in a cell, wherein the method comprises contacting said cell with a protein capable of modifying a nucleic acid and a transduction buffer, wherein the transduction buffer comprises (i) a transduction compound selected from compounds 42, 31, 45, 43, 34, 41, 40, 39, 33, 44, 22, 20, 03, 30, 15, 38, 35, 11, 10, 28, 37, 01, 29, 02, 21, 36, 26, 16, 04, 19, 23, 24, 12, 14, 13, 05, 08 and 27 in Table 1, and (ii) a salt selected from a sodium, lithium, potassium, caesium or rubidium salt, or an activator/enhancer of a sodium-hydrogen transporter, wherein the transduction buffer has an osmolality of at least 750 mOsm/kg and a pH of between about 6 and about 8, thereby transducing the protein into said cell and creating a modified cell.
Dependencies are inferred from the claim text.