Plants producing 2n gametes or apomeiotic gametes
AssigneeInstitut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement
InventorsRaphael Mercier, Isabelle D'Erfurth, Nicole Froger, Sylvie Jolivet, Laurence Cromer
Life of this patent
Estimating the term…
Abstract
The invention relates to plants wherein the protein OSD1, involved in the transition from meiosis I to meiosis II is inactive. These plants produce Second Division Restitution (SDR) 2n gametes. The invention further relates to plants wherein the inactivation of OSD1 is combined with the inactivation of a gene involved in meiotic recombination in plants, and of a gene involved in the monopolar orientation of the kinetochores during meiosis. These plants produce apomeiotic gametes. These plants are useful in plant breeding.
Claims13
A method for obtaining a plant producing Second Division Restitution 2n gametes, comprising
- inhibiting in said plant an Omission of Second Division 1 protein (OSD1),
- wherein said plant is rice,
- wherein said OSD1 protein has
- at least 90% sequence identity with the OSD1 protein from Oryza sativa (0s0SD1) as set forth in SEQ ID NO:
- 35, and
- wherein said protein allows a second meiotic division to occur, and thereby is necessary for the transition from meiosis I to meiosis II,
- wherein the inhibition of the OSD1 protein is obtained by
- (i) mutating an OSD1 gene or its promoter and selecting a mutant plant having partially or totally lost OSD1 protein activity, or
- (ii) expressing a silencing RNA targeting the OSD1 gene encoding said OSD1 protein in said plant,
- thereby obtaining a rice plant that forms dyads.
A method for obtaining a plant producing apomeiotic gametes,
- wherein said plant is rice,
- wherein said method comprises an inhibition in said plant of the following native plant proteins:
- (a) an Omission of Second Division 1 protein (OSD1) havinq at least 90% sequence identity with the OSD1 protein from Oryza sativa (0s0SD1) as set forth in SEQ ID NO:
- 35;
- and
- (b) a plant protein involved in initiation of meiotic recombination in plants, said protein being selected among:
- (i) a plant sporulation 11-1 (SP011-1) protein, wherein said protein has at least 40% sequence identity with the SP011-1 protein of SEQ ID NO:
- 2;
- (ii) a plant sporulation 11-2 (SP011-2) protein, wherein said protein has at least 40% sequence identity with the SP011-2 protein of SEQ ID NO:
- 3;
- (iii) a plant putative recombination initiation defect 1 (PRD1) protein, wherein said protein has at least 25% sequence identity with the PRD1 protein of SEQ ID NO:
- 4;
- or
- (iv) a plant homologous pairing aberration in rice 1 (PAIR1) protein, wherein said protein has at least 30% sequence identity with the PAIR1 protein of SEQ ID NO:
- 5;
- and
- (c) a plant meiotic recombination protein 8 (Rec8) protein, wherein said protein has at least 40% sequence identity with the Rec8 protein of SEQ ID NO:
- 6, wherein the inhibition of at least one of the OSD1, SP011-1, SPO11-2, PRD1, PAIR1, or Rec8 proteins is obtained by
- (i) mutating a gene encoding said protein or its promoter and selecting a mutant plant having partially or totally lost an activity of said protein, or
- (ii) expressing in said plant a silencing RNA targeting the gene encoding said protein.
A method for obtaining a plant producing Second Division Restitution 2n gametes, comprising inhibiting in said plant an Omission of Second Division 1 protein (OSD1),
- wherein said plant is rice,
- wherein said OSD1 protein is the OSD1 protein from Oryza sativa (OsOSD1) as set forth in SEQ ID NO:
- 35,
- wherein said OSD1 protein allows a second meiotic division to occur, and thereby is necessary for the transition from meiosis I to meiosis II, and
- wherein the inhibition of said OsOSD1 protein is obtained by
- (i) mutating an OsOSD1 gene or its promoter and selecting a mutant plant having partially or totally lost OsOSD1 protein activity, or
- (ii) expressing a silencing RNA targeting the OsOSD1 gene encoding said OsOSD1 protein in said plant,
- thereby obtaining a rice plant that forms dyads.
Dependencies are inferred from the claim text.