Nucleic acid constructs for genome editing
AssigneeDanziger Innovations Ltd.
InventorsAlexander Vainstein, Ira Marton, Arik Honig, Elena Marhevka
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Abstract
A nucleic acid construct is provided. The construct comprises a tobacco rattle virus (TRV) sequence and a nucleic acid sequence encoding a single guide RNA (sgRNA) that mediates sequence-specific cleavage in a target sequence of a genome of interest, wherein the TRV sequence is devoid of a functional 2b sequence. Also provided are plant cells comprising the construct and uses of the construct in gene editing.
Claims21
A nucleic acid construct comprising or encoding a nucleic acid sequence of a tobacco rattle virus (TRV) RNA2 sequence and a single guide RNA (sgRNA) that mediates sequence-specific cleavage in a target sequence of a genome of interest, wherein said TRV RNA2 sequence is devoid of a functional 2b sequence, wherein said TRV RNA2 is devoid of a functional coat protein and wherein said nucleic acid construct replicates and spreads as an RNA virus in a plant.
Dependencies are inferred from the claim text.