Fermentative glycerol-free ethanol production
AssigneeDsm IP Assets B.V.
InventorsJacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Abstract
The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
Claims30
Transgenic yeast cells comprising:
- a recombinant heterologous nucleic acid sequence encoding a protein comprising NAD+-dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10) and NAD+-dependent alcohol dehydrogenase activity (EC 1.1.1.1), a nucleic acid sequence encoding a protein with acetyl-Coenzyme A synthetase activity (EC 6.2.1.1), and
- a nucleic acid sequence encoding a protein with NADH-dependent glycerol-3-phosphate dehydrogenase activity (EC 1.1.1.8);
- wherein the transgenic yeast cells reduce the amount of glycerol in spent medium of an ethanol-producing fermentation process as compared to their corresponding wild-type yeast cells.
Transgenic S. cerevisiae yeast cells comprising:
- a recombinant heterologous nucleic acid sequence encoding a protein comprising an adhE enzyme with NAD+-dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10) and NAD+-dependent alcohol dehydrogenase activity (EC 1.1.1.1),
- a nucleic acid sequence encoding a protein with acetyl-Coenzyme A synthetase activity (EC 6.2.1.1), and
- a nucleic acid sequence encoding a protein with NADH-dependent glycerol-3-phosphate dehydrogenase activity (EC 1.1.1.8);
- wherein the transgenic yeast cells exhibit reduced glycerol content in spent medium of an ethanol-producing fermentation process as compared to their corresponding wild-type yeast cells.
Dependencies are inferred from the claim text.