Rotationally sequestered translators
AssigneeEmerald Therapeutics, Inc.
InventorsDaniel J. Kleinbaum, Brian M. Frezza, Brad Bond, Jonathan Leung, George W. Fraser
Life of this patent
Estimating the term…
Abstract
Provided are nucleic acid translators capable of carrying out logic operations with improved efficiency, maximized output and reduced off-target effects, in particular in a biological system. Methods of using these translators to transduce signal are also provided.
Claims9
A composition comprising a first nucleic acid complex comprising (a) a first, a second, a third and a fourth nucleic acid strands each comprising, sequentially, a first, a second and a third fragments and (b) a first and second RNA strands, wherein the first complex comprises:
- (i) a first duplex region formed between the second fragments of the first and second strands;
- (ii) a second duplex region formed between the second fragments of the third and fourth strands;
- (iii) a third duplex region formed between the third fragment of the first strand and the first fragment of the third strand;
- (iv) a fourth duplex region formed between the first fragment of the second strand and the third fragment of the fourth strand;
- (v) a fifth duplex region formed between the third fragment of the third strand and the first RNA;
- and
- (vi) a sixth duplex region formed between the first fragment of the fourth strand and the second RNA,
- and the first fragment (C) of the first strand and the third fragment (D) of the second strand are single-stranded.
A nucleic acid complex comprising a first, a second, a third, a fourth, a fifth and a sixth nucleic acid strands, wherein:
- the first strand comprises, sequentially, a first, a second, a third and a fourth fragments and is defined as Q-X-B-Ā, wherein each letter denotes a fragment and a string of letters connected by “-” denotes a strand;
- the second strand comprises, sequentially, a first, a second, a third and a fourth fragments and is defined as B-Ā-X-P;
- the third and fifth strands each comprises, sequentially, a first, a second and a third fragments and is defined as A-Y-C;
- and
- the fourth and sixth strands each comprises, sequentially, a first, a second and a third fragments and is defined as D-Y-B;
- and wherein the complex comprises:
- a first duplex region (X:X) formed between the second fragment of the first strand and the third strand of the second strand;
- a second duplex region (Ā::A) formed between the fourth fragment of the first strand and the first strand of the third strand;
- a third duplex region (B::B) formed between the third fragment of the first strand and the third fragment of the fourth strand;
- a fourth duplex region (Ā::A) formed between the second fragment of the second strand and the first fragment of the fifth strand;
- a fifth duplex region (B::B) formed between the first fragment of the second strand and the third fragment of the sixth strand;
- a sixth duplex region (Y::Y) formed between the second fragments of the third and fourth strands;
- and
- a seventh duplex region (Y::Y) formed between the second fragments of the fifth and sixth strands,
- and wherein the first fragment (Q) of the first strand, the fourth fragment (P) of the second strand, the third fragments (C) of the third and fifth strands and the first fragments (D) of the fourth and sixth strands are single-stranded.
Dependencies are inferred from the claim text.