Compositions and methods for delivery of a polynucleotide into a plant
AssigneeMonsanto Technology LLC
InventorsMichael J. Bennett, Shihshieh Huang, Alberto B. Iandolino, Heather A. Lindfors, Janette V. Oakes, Gregory John Peel, Sharon E. Radke, Steven H. Schwartz
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Abstract
The present disclosure provides compositions and methods for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell. More specifically, the present disclosure relates to compositions comprising at least one polynucleotide and at least one agent that is able to disrupt at least one barrier of the plant or plant part.
Claims8
A method for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell, comprising
- a) applying onto the surface of the plant or plant part at least one agent that is able to disrupt at least one barrier of the plant or plant part, and
- b) applying onto the surface of the plant or plant part one or more polynucleotides,
- wherein the at least one agent comprises a lipase enzyme,
- wherein steps a) and b) are carried out concurrently or sequentially in any order, and
- wherein the method further comprises applying onto the surface of the plant or plant part one or more osmolytes selected from the group consisting of sorbitol, mannitol, D-proline, and L-proline.
Dependencies are inferred from the claim text.