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US 10,883,094 B2Granted
Transposase polypeptides and uses thereof
AssigneeBoard of Regents, The University of Texas System
InventorsLaurence J. N. Cooper, Natalya Belousova
Life of this patent
Estimating the term…
Abstract
Transposase polypeptides and polynucleotides are provided, which have a high activity in mammalian cells. Methods for engineering cells, such as chimeric antigen T-cells, with the transposes are also provided.
Claims19
A method of providing an immune response in a human subject in need thereof comprising:
- (a) transfecting an immune cell with
- (i) a nucleic acid encoding a recombinant polypeptide having transposase activity, wherein said polypeptide comprises an amino acid sequence at least 90% identical to the sequence of SEQ ID NO:
- 1, wherein the polypeptide comprises:
- (i) an Arg at the position corresponding to position 14;
- (ii) an Ala at the position corresponding to position 33;
- (iii) a His at the position corresponding to position 115;
- (iv) an Arg at the position corresponding to position 136;
- (v) an Asp at the position corresponding to position 214;
- (vi) an Ala at the position corresponding to position 215;
- (vii) a Val at the position corresponding to position 216;
- (viii) a Gln at the position corresponding to position 217;
- (ix) a His at the position corresponding to position 243;
- (x) a His at the position corresponding to position 253;
- and
- (xi) an Arg at the position corresponding to position 255;
- and
- (ii) a DNA encoding a CAR or TCR flanked by transposon repeats;
- and
- (b) administering the transfected immune cells expressing a CAR or TCR to the subject to provide a T-cell response.
16 more elements
A method of treating a human subject having a disease cancer comprising:
- (a) transfecting an immune cell with
- (i) a nucleic acid encoding a recombinant polypeptide having transposase activity, wherein said polypeptide comprises an amino acid sequence at least 90% identical to SEQ ID NO:
- 1, wherein the polypeptide comprises:
- (i) an Arg at the position corresponding to position 14;
- (ii) an Ala at the position corresponding to position 33;
- (iii) a His at the position corresponding to position 115;
- (iv) an Arg at the position corresponding to position 136;
- (v) an Asp at the position corresponding to position 214;
- (vi) an Ala at the position corresponding to position 215;
- (vii) a Val at the position corresponding to position 216;
- (viii) a Gln at the position corresponding to position 217;
- (ix) a His at the position corresponding to position 243;
- (x) a His at the position corresponding to position 253;
- and
- (xi) an Arg at the position corresponding to position 255;
- and
- (ii) a DNA encoding a CAR or TCR flanked by transposon repeats;
- and
- (b) administering a therapeutically effective amount of the transfected immune cells expressing a CAR or TCR to the subject, thereby effecting an anti-tumor response to reduce or eliminate tumors in the subject.
16 more elements
Dependencies are inferred from the claim text.